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| Registros recuperados: 24 | |
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| Robert, Rene; Chretiennot-dinet, Marie-josèphe; Kaas, Raymond; Martin-jezequel, Véronique; Moal, Jeanne; Le Coz, Jean-rene; Nicolas, Jean-louis; Bernard, Eudes; Connan, Jean-paul; Le Dean, Loic; Le Gourrierec, Gaetane; Leroy, Bertrand; Quere, Claudie. |
| in mollusc hatcheries. Consequently, we assessed themost commonly used species to establish their growth and size profiles, their cytomorphological, biochemical, biomolecular identification as well as their cytometer signs, and also their ability to grow under extreme conditions of temperature and salinity. This work aimed at matching the conformity of the strains used in Argenton (dispatched to most of the French commercial hatcheries) to those of a referenced culture collection. A previous screening preceded this study, because of the 45 species and/or clones held originally, Argenton kept no more than 15 species. As most of the latter are also represented in referenced collection, our strains are therefore correctly identified. On the other hand, the... |
| Tipo: Text |
Palavras-chave: Ecological limits; Biomolecular profile; Flow cytometry; Biochemical composition; Cytomorphology; Phytoplankton; Mollusc; Hatchery; Ecotolérance; Profil biomoléculaire; Cytométrie en flux; Composition biochimique; Cytomorphologie; Phytoplancton; Mollusques; Ecloserie. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/rapport-1546.pdf |
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| Marie, Benjamin; Joubert, Caroline; Belliard, Corinne; Tayale, Alexandre; Zanella-cleon, Isabelle; Marin, Frederic; Gueguen, Yannick; Montagnani, Caroline. |
| Nacre of the Pinctada pearl oyster shells is composed of 98% CaCO3 and 2% organic matrix. The relationship between the organic matrix and the mechanism of nacre formation currently constitutes the main focus regarding the biomineralization process. In this study, we isolated a new nacre matrix protein in P. margaritifera and P. maxima, we called Pmarg- and Pmax-MRNP34 (methionine-rich nacre protein). MRNP34 is a secreted hydrophobic protein, which is remarkably rich in methionine, and which is specifically localised in mineralizing the epithelium cells of the mantle and in the nacre matrix. The structure of this protein is drastically different from those of the other nacre proteins already described. This unusual methionine-rich protein is a new member in... |
| Tipo: Text |
Palavras-chave: Matrix protein; Methionine-rich; Biomineralization; Mollusc; Nacre; Calcifying mantle. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00077/18870/16645.pdf |
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| Hanquet, Anne-caroline; Kellner, K; Heude, C; Naimi, Amine; Mathieu, M; Poncet, J.m.. |
| Cryopreservation is widely used for long-term conservation of various tissues, embryos or gametes. However, few studies have described cryopreservation of invertebrate primary cell cultures and more particularly of marine invertebrate somatic cells. This technique would however be of great interest to facilitate the study of various metabolic processes which vary seasonally. The aim of the present study was to develop a protocol for cryopreservation of Crassostrea gigas vesicular cells. Different parameters were adjusted to improve recovery of cells after freezing. The most efficient cryoprotectant agent was a mix of Me2SO, glycerol, and ethylene glycol (4% each). The optimal cooling rate was -1 degrees C min(-1) down to -70 degrees C before transfer into... |
| Tipo: Text |
Palavras-chave: Glycogen metabolism; Vesicular cells; Cryopreservation; Mollusc; Oyster; Crassostrea gigas. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1938.pdf |
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| Botana, Luis M.; Hess, Philipp; Munday, Rex; Nathalie, Arnich; Degrasse, Stacey L.; Feeley, Mark; Suzuki, Toshiyuki; Van Den Berg, Martin; Fattori, Vittorio; Gamarro, Esther Garrido; Tritscher, Angelika; Nakagawa, Rei; Karunasagar, Iddya. |
| Background Seafood toxins pose an important risk to human health, and maximum levels were imposed by regulatory authorities throughout the world. Several toxin groups are known, each one with many analogues of the major toxin. Regulatory limits are set to ensure that commercially available seafood is not contaminated with unsafe levels. Scope and Approach The mouse bioassay was used to measure the toxicity in seafood extracts to determine if a sample exceeded regulatory limits. The advantage of this approach was to provide an estimation of the total toxicity in the sample. As instrumental methods of analysis advance and serve as replacements to the mouse bioassay, the challenge is translating individual toxin concentrations into toxicity to determine... |
| Tipo: Text |
Palavras-chave: Marine toxins; Toxicity Equivalency Factors; FAO; WHO; Bivalve; Mollusc. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00358/46960/46874.pdf |
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| Roussel, Sabine; Poitevin, Pierre; Day, Robert; Le Grand, Fabienne; Stiger‐pouvreau, Valérie; Leblanc, Catherine; Huchette, Sylvain. |
| While population foraging behaviour of herbivores has been extensively studied, individual choice is still poorly understood. Very few studies have focused on the individual consistency of foraging behaviour in marine herbivores. Because marine ectotherms are strongly influenced by their environment and because a mixed diet is appropriate for herbivores, we hypothesized that Haliotis tuberculata, a large marine gastropod, would not exhibit significant individual consistency in foraging activity and would display generalist food choices. To test these hypotheses, the behaviour of 120 abalone was studied using a choice test of eight macroalgal species over 3 weeks, with video recording 24 hr a day. In addition, primary components, secondary metabolites and... |
| Tipo: Text |
Palavras-chave: Food choice; Foraging activity; Individual consistency; Intraclass correlation; Marine herbivore; Mollusc; Proportion similarity index. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00613/72465/71613.pdf |
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| Trinkler, Nolwenn; Bardeau, Jean-francois; Marin, Frederic; Labonne, Maylis; Jolivet, Aurelie; Crassous, Philippe; Paillard, Christine. |
| The mineral phase of shell repair in the Manila clam Venerupis philippinarum affected by brown ring disease (BRD) was characterised at various scales and at various stages of shell repair by confocal Raman microspectrometry and scanning electron microscopy. Spherulitic and quadrangular aragonite microstructures associated with polyene pigments were clearly observed. Von Kossa staining showed that at the beginning of shell repair, hemocytes are filled with insoluble calcium carbonate salts in all fluids and then are transported toward the extrapallial fluids and the repair sites. Our analyses suggest that after a Vibrio tapetis attack and BRD deposit some clams rapidly cover the deposit, resulting in a modification in the microstructure, which could be... |
| Tipo: Text |
Palavras-chave: Aragonite; Hemocytes; Shell microstructure; Calcium carbonate; Biomineralization; Mollusc; Raman microspectrometry; Scanning electron microscopy. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00029/14065/11301.pdf |
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| Tanguy, Arnaud; Boutet, Isabelle; Boudry, Pierre; Degremont, Lionel; Laroche, Jean; Moraga, Dario. |
| Phosphoglucomutase is a key enzyme in glycolysis and has been widely studied in vertebrates and some invertebrates but no molecular information is available in marine invertebrates despite the importance of this marker in ecological and genetical studies. In this work, we isolated a cDNA and the corresponding genomic sequence that encode PGM-2 locus in the Pacific oyster Crassostrea gigas. We used sequences drawn from the database to construct an evolutionary framework for examining the position of mollusc PGM sequences among prokaryotic and eukaryotic homologues and showed that oyster PGM gene organization was closer to vertebrates PGM genes than other invertebrates as previously found in other Lophotrochozoa species. We also investigated PGM mRNA... |
| Tipo: Text |
Palavras-chave: Gene expression; Xenobiotics; Mollusc; Phosphoglucomutase. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2220.pdf |
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| Suquet, Marc; Malo, Florent; Gourtay, Clemence; Ratiskol, Dominique; Riobe, Marion; Le Grand, Jacqueline; Queau, Isabelle; Mingant, Christian. |
| A basic cryopreservation protocol for great scallop sperm was recently published, with which thawed sperm survival remained limited to 25% of control values (Suquet et al. 2014). We designed here a set of five experiments to test ways of improving thawed sperm survival and storage by studying the effects of (1) cryoprotectant concentration; (2) sugar incorporation in the extender; (3) egg yolk addition to the extender; (4) sperm dilution rate; and (5) storage capacity of thawed sperm at 4 degrees C. A sixth experiment was then performed to compare rearing performances of larvae produced using thawed sperm from the optimal protocol defined in the present study with those produced using fresh sperm. During the first experiment, a significantly higher... |
| Tipo: Text |
Palavras-chave: Pecten maximus; Mollusc; Sperm; Cryopreservation; Larval rearing. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00359/47033/46946.pdf |
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| Guichard, Benjamin; Garcia, Celine; Joly, Jean-pierre; Lupo, Coralie; Chollet, Bruno; Robert, Maeva; Omnes, Emmanuelle; Arzul, Isabelle. |
| The REPAMO is a French Network for the surveillance of Mollusc diseases that notably meets the obligations of the European Directive 2006/088/EC. It is entirely funded by the French State. The coordination of this network entrusted to the Institut Français de Recherche pour l`Exploitation de la Mer since 1986 aims at monitoring the notifiable endemic diseases, detecting the emergence of exotic or new pathogens and ensuring a minimum level of surveillance of the general health condition of molluscs. Beside IFREMER, different partners are involved in the surveillance of mollusc health at the national level: the Departmental direction for territories and sea who are the local representatives of the competent authority and the producers who have the obligation... |
| Tipo: Text |
Palavras-chave: Surveillance; Mollusc; Pathology; Network. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00036/14768/12077.pdf |
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| Le Quere, Herve; Herpin, Amaury; Huvet, Arnaud; Lelong, Christophe; Favrel, Pascal. |
| Members of the Transforming Growth factor beta (TGF-beta) superfamily of cell signalling polypeptides are known to play important roles in cell proliferation and differentiation during development and in various physiological processes of most animal clades. Recent findings in the mollusc Crassostrea gigas demonstrate the occurrence of a diversity of TGF-beta signalling components including various ligands, three type I receptors but only a single type II receptor. This report describes the characterization of Cg-ActRII, a new type II receptor displaying homology with vertebrate and Drosophila Activin type II receptors. The use of zebrafish embryo as a reporter organism revealed that, in a way similar to its zebrafish counterpart, overexpression of... |
| Tipo: Text |
Palavras-chave: Activin receptor; Nervous tissues; Development; Lophotrochozoa; Mollusc. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6401.pdf |
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| Registros recuperados: 24 | |
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